Repository navigation
Add SMARTer smRNA kit support and standalone RNA-seq summary reports - #6
Merged
Merged
Conversation
This file contains hidden or bidirectional Unicode text that may be interpreted or compiled differently than what appears below. To review, open the file in an editor that reveals hidden Unicode characters.
Learn more about bidirectional Unicode characters
Sign up for free
to join this conversation on GitHub.
Already have an account?
Sign in to comment
Add this suggestion to a batch that can be applied as a single commit.This suggestion is invalid because no changes were made to the code.Suggestions cannot be applied while the pull request is closed.Suggestions cannot be applied while viewing a subset of changes.Only one suggestion per line can be applied in a batch.Add this suggestion to a batch that can be applied as a single commit.Applying suggestions on deleted lines is not supported.You must change the existing code in this line in order to create a valid suggestion.Outdated suggestions cannot be applied.This suggestion has been applied or marked resolved.Suggestions cannot be applied from pending reviews.Suggestions cannot be applied on multi-line comments.Suggestions cannot be applied while the pull request is queued to merge.Suggestion cannot be applied right now. Please check back later.
SMARTer smRNA libraries need protocol-specific trimming and must bypass the Total RNA UMI workflow. This change adds a selectable smRNA profile while preserving
smarter_total_rnaas the default, and produces a separate concise RNA-seq summary alongside the comprehensive report.Changes
--kit smarter_smrnafor single-end R1 or paired-end R1/R2 input, with sense/forward orientation by default, structure/poly(A) trimming, Q20 filtering, and no UMI extraction or deduplication.